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- Title
Evidence of finely tuned expression of DNA polymerase β in vivo using transgenic mice
- Authors
Bergoglio, Valérie; Fréchet, Mathilde; Philippe, Magali; Bieth, Anne; Mercier, Pascale; Morello, Dominique; Lacroix-Tricki, Magali; Delsol, Georges; Hoffmann, Jean-Sébastien; Cazaux, Christophe
- Abstract
DNA polymerase β (Pol β) is an error-prone repair DNA polymerase that has been shown to create genetic instability and tumorigenesis when overexpressed by only 2-fold in cells, suggesting that a rigorous regulation of its expression may be essential in vivo. To address this question, we have generated mice which express a transgene (Tg) bearing the Pol <f>β</f> cDNA under the control of the ubiquitous promoter of the mouse H-2K gene from the major histocompatibility complex. These mice express the Tg only in thymus, an organ which normally contains the most abundant endogenous Pol <f>β</f> mRNA and protein, supporting the idea of a tight regulation of Pol β in vivo. Furthermore, we found no tumor incidence, suggesting that the single Pol β overexpression event is not sufficient to initiate tumorigenesis in vivo.
- Subjects
DNA polymerases; TRANSGENIC mice; TRANSGENIC animals; TRANSCRIPTION factors
- Publication
FEBS Letters, 2004, Vol 566, Issue 1-3, p147
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.04.020