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- Title
Dexamethasone treatment specifically increases the basal proton conductance of rat liver mitochondria
- Authors
Roussel, Damien; Dumas, Jean-François; Augeraud, Antoine; Douay, Olivier; Foussard, Françoise; Malthiéry, Yves; Simard, Gilles; Ritz, Patrick
- Abstract
We investigated the role that mitochondrial proton leak may play in the glucocorticoid-induced hypermetabolic state. Sprague–Dawley rats were injected with dexamethasone over a period of 5 days. Liver mitochondria and gastrocnemius subsarcolemmal and intermyofibrillar mitochondria were isolated from dexamethasone-treated, pair-fed and control rats. Respiration and membrane potential were measured simultaneously using electrodes sensitive to oxygen and to the potential-dependent probe triphenylmethylphosphonium, respectively. Five days of dexamethasone injection resulted in a marked increase in the basal proton conductance of liver mitochondria, but not in the muscle mitochondrial populations. This effect would have a modest impact on energy expenditure in rats.
- Subjects
GLUCOCORTICOIDS; MITOCHONDRIAL pathology
- Publication
FEBS Letters, 2003, Vol 541, Issue 1-3, p75
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00307-7