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- Title
Macrophage migration inhibitory factor expression correlates with inflammatory changes in human chronic hepatitis B infection.
- Authors
Zhang, Hai-Ying; Nanji, Amin A.; Luk, John M.; Huang, Xiao-Ru; Lo, Chung-Mau; Chen, Yong Xiong; Yuen, Siu-Tsan; Lan, Hui Y.; Lau, George K. K.
- Abstract
Zhang H-Y, Nanji AA, Luk JM, Huang X-R, Lo C-M, Chen YX, Yuen S-T, Lan HY, Lau GKK. Macrophage migration inhibitory factor expression correlates with inflammatory changes in human chronic hepatitis B infection.Liver International: 2005: 25: 571–579.© Blackwell Munksgaard 2005Macrophage migration inhibitory factor (MIF) has emerged to be a pivotal cytokine in immune-mediated diseases.To investigate the role of MIF in chronic hepatitis B infection, we studied two groups of hepatitis B surface antigen positive patients: group 1 (immune tolerant,n=16) and group 2 (immune clearance,n=16). Serum level of MIF was measured by enzyme-linked immunosorbent assay and intrahepatic expression of MIF, macrophage and T-cell localisation were detected by double immunohistochemistry.An increased serum MIF correlated significantly with increased serum alanine aminotransferase activity (r=0.73,P<0.001) and the severity of necroinflammatory injury (r=0.642,P<0.001). In group 2, there was marked MIF mRNA expression in all KP-1+ macrophages and CD45RO+ activated T cells and, to a lesser extent, in hepatocytes within inflammatory areas. In contrast to its mRNA expression, the cytoplasmic MIF protein level in hepatocytes, infiltrating macrophages and T cells within the inflammatory area was reduced, which probably contributed to the increased serum MIF level.Our data suggested that MIF played a role in sustaining cell-mediated hepatic injury during the immune-clearance phase of chronic hepatitis B infection.
- Subjects
HEPATITIS B; MACROPHAGES; MESSENGER RNA; LIVER diseases; BLOOD plasma; ENZYME-linked immunosorbent assay
- Publication
Liver International, 2005, Vol 25, Issue 3, p571
- ISSN
1478-3223
- Publication type
Article
- DOI
10.1111/j.1478-3231.2005.01047.x