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- Title
The active ingredient (DSH-20) of Salvia miltiorrhiza flower reduces oxidative damage and apoptosis in cardiomyocytes by regulating miR-1.
- Authors
Cao, Panxia; Xue, Yanpin; Guo, Mengjiao; Jiang, Xue; Lei, Zhen; Gao, Shuibo; Wang, Xinzhou; Gao, Haixia; Han, Yongjun; Chang, Hongbo; Liu, Shanshan; Dai, Liping; Wu, Hong
- Abstract
Background: DSH-20, the active ingredient of Salvia miltiorrhiza flower extract, is used to treat cardiovascular diseases. However, its mechanism of action remains unclear. Herein, we investigated the intervention of DSH-20 in H2O2-induced oxidative damage and apoptosis in cardiomyocytes. Methods and Results: H2O2 was used to induce oxidative damage and apoptosis in H9c2 cardiomyocytes. Based on concentration gradient studies, we found that 62.5 µg/mL DSH-20 significantly reduced reactive oxygen species and lactate dehydrogenase levels and increased superoxide dismutase levels. DSH-20 also alleviated the apoptosis rate, the changes in mRNA of apoptosis-related genes (Bcl-2, BAX, and Caspase-3) and miR-1 expression. Moreover, transfection of miR-1 mimics aggravated oxidative damage and apoptosis, whereas DSH-20 alleviated these effects. Conclusions: DSH-20 reduced H2O2-induced oxidative damage and apoptosis in H9c2 cardiomyocytes likely by downregulating miR-1 expression.
- Subjects
SALVIA miltiorrhiza; LACTATE dehydrogenase; SUPEROXIDE dismutase; CONCENTRATION gradient
- Publication
Molecular Biology Reports, 2022, Vol 49, Issue 5, p3675
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-022-07207-5