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- Title
Isolation, characterization and quantitation of canine alpha-1-acid glycoprotein.
- Authors
M. Yuki; H. Itoh; K. Tamura; N. Nishii; K. Takase
- Abstract
Abstract The present study was designed to investigate the physicochemical properties of canine alpha-1-acid glycoprotein (AGP), and to establish a method for measuring serum AGP in canine. Fifty-three normal beagles were used in the study. AGP was purified from normal canine sera by successive ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. The serum AGP concentration was measured by single radial immunodiffusion (SRID). Canine AGP had a molecular weight of 42,000 ± 2,000 Da and contained 40.9% carbohydrate. Gel isoelectric focusing revealed microheterogeneity with 6–7 bands in a pI range (isoelectric points) of 3.2–3.8. AGP migrated to the α1-globulin region on immunoelectrophoresis. The serum AGP level of 35 normal beagles was 283.4 ± 113.3 μg/ml. Canine AGP was isolated, and its physicochemical properties were clarified. SRID may be a useful method for quantification of serum AGP in canine.
- Subjects
GLYCOPROTEINS; BLOOD proteins; BEAGLE (Dog breed); ION exchange chromatography; IMMUNODIFFUSION; MOLECULAR weights; PHYSIOLOGY
- Publication
Veterinary Research Communications, 2008, Vol 32, Issue 7, p533
- ISSN
0165-7380
- Publication type
Article
- DOI
10.1007/s11259-008-9055-9