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- Title
Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced RANTES transcription in PK-15 cells.
- Authors
Dang Wang; Liurong Fang; Jing Bi; Quangang Chen; Lu Cao; Rui Luo; Huanchun Chen; Shaobo Xiao
- Abstract
The chemokine RANTES (regulated upon activation, normal T-cells expressed and secreted) plays an essential role in inflammation and immune response. Infection with wild-type foot-and-mouth disease virus (FMDV) in PK-15 cells strongly inhibits the expression of RANTES compared to infection with a genetically engineered mutant lacking the leader protein (L) coding region. This suggests that L is involved in RANTES regulation. However, the underlying molecular mechanism remains unclear. In this study, we show that transfection of PK-15 cells with a plasmid expressing the L of FMDV, in the absence of other FMDV proteins, inhibited dsRNA-induced RANTES transcription and promoter activity. Promoter mutagenesis experiments revealed that the interferon-stimulated response element (ISRE) was important for the ability of L to inhibit dsRNA-induced RANTES promoter activity. Furthermore, over-expression of L also inhibited IRF-3/7-mediated RANTES activation. Screening L mutants indicated that catalytic activity and a SAP (for SAF-A/B, Acinus, and PIAS) domain of L were required to suppress dsRNA-induced RANTES transcription.
- Subjects
FOOT &; mouth disease virus; PROTEINASES; CHEMOKINES; GENE transfection; GENETIC transcription; GENETIC engineering
- Publication
Virus Genes, 2011, Vol 42, Issue 3, p388
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-011-0590-z