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- Title
Purification and characterization of a high salt-tolerant alginate lyase from Cobetia sp. WG-007.
- Authors
Gong, Jin‐Song; Liu, Xu‐Mei; Zhang, Ming‐Jie; Li, Heng; Geng, Yan; Li, Hui; Li, Jing; Lu, Zhen‐Ming; Xu, Zheng‐Hong; Shi, Jin‐Song
- Abstract
An alginate lyase producing bacterial strain, Cobetia sp. WG-007, was isolated and identified from rotting seaweed. The alginate lyase, Aly-W02, was purified by procedures of ultrafiltration, Q-Sepharose Fast Flow, Phenyl Sepharose 6 Fast Flow, and Superdex-G100 with specific activity of 21,285.5 U/mg. Aly-W02 had an apparent molecular mass of 35 kDa. It exhibited maximum activity at 45 °C in 50 mM sodium phosphate buffer (pH 8.5). This alginate lyase was stable in the pH range of 6.0-8.5. Among the tested metal ions, the addition of K+, Na+, and Mg2+ ions can enhance the enzyme activities, while Ba2+, Ni+, Cu2+, Mn2+, Zn2+, Ag+, and ethylenediaminetetraacetic acid decreased the activities. It displayed high salt-tolerant ability; 0.8 M NaCl or 1.5 M KCl significantly enhanced the enzyme activity. Furthermore, Aly-W02 mainly released disaccharide, trisaccharide, and tetrasaccharid from alginate. It showed potential in producing low molecular weight alginate oligosaccharides.
- Subjects
ALGINATE lyase; SEPHAROSE; AGAR; GEL permeation chromatography; IMMUNOELECTROPHORESIS; ULTRAFILTRATION
- Publication
Biotechnology & Applied Biochemistry, 2017, Vol 64, Issue 4, p519
- ISSN
0885-4513
- Publication type
Article
- DOI
10.1002/bab.1506