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- Title
A Rapid and Efficient System of Agrobacterium Infection-Mediated Transient Gene Expression in Rice Oc Cells and Its Application for Analysis of the Expression and Antisense Suppression of Preprophytosulfokine, a Precursor of Phytosulfokine-α, Encoded ...
- Authors
Yang, Heping; Morita, Akiko; Matsubayashi, Yoshikatsu; Nakamura, Kenzo; Sakagami, Youji
- Abstract
A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-α, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression.
- Subjects
AGROBACTERIUM; BACTERIAL diseases of plants; GENE expression in plants; RICE; ANTISENSE genetics; MICROBIOLOGY; PLANTS; PLANT cells &; tissues
- Publication
Plant & Cell Physiology, 2000, Vol 41, Issue 6, p811
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/41.6.811