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- Title
Isolation and Screening of Indigenous Rhizobia from BlackGram Cultivated in Fallow Rice Soils for Plant Growth Promoting Traits.
- Authors
Satyanandam, T.; Babu, K.; Suneeta, D.; Bhaskararao, C. H.; Rosaiah, G.; Vijayalakshmi, M.
- Abstract
Bio fertilisers are relatively safer, environmentally friendly and a cost-effective approach to chemical fertiliser usage. The selection of bacterial strains with multiple beneficial characteristics is important to maximise their effectiveness on the host plant. In the present study, four native and indigenous rhizobial strains (VM-2, VM-8, VM-9 and VM-15) were isolated from root nodules of blackgram (Vignamungo) cultivated in fallow rice soils of Andhra Pradesh, India. All the four strains were screened in vitro for their plant growth-promoting (PGP) characteristics viz. production of indole acetic acid (IAA), exopolysaccharide (EPS), hydrogen cyanide (HCN) and phosphate solubilisation. The results indicated that the rhizobial strains varied in their plant growth promoting activities. All the four strains produced IAA, EPS and also solubilised the insoluble phosphate. The amount of IAA produced varied from strain to strain and relatively high amounts were recorded in VM-8 (43.4 µg/ml) followed by VM-15 with 43.1 µg/ml. Maximum EPS production was recorded in VM-9 (527 mg/ml) followed by VM-8 (483 mg/ml). The phosphate solubilisation efficiency of Rhizobium strains on solid media ranged between 16% and 17%. In liquid medium, strain VM-2 recorded maximum solubilisation (799µg/ml) followed by VM-8 (372µg/ml). All the strains except strain VM-8 were HCN producers. Among these three strains, VM-2 and VM-15 showed strong HCN production. These isolates were identified as Rhizobium sp. strain VM-2 (KJ 704783), Brady rhizobium sp. strain VM-8 (KJ 704784), Brady rhizobium sp. strain VM-9 (KJ 704785) and Achromobacter sp. strain VM-15 (KJ501696) after 16S rRNA sequencing. The pot culture experiment showed that VM-8, VM-9 and VM-15 inoculated plants had good results both in inoculated sterilised and inoculated unsterilised soils than the plants grown in sterilised uninoculated soils and control soils. The VM-2 strain showed moderate results under plant inoculation test. This study suggests that these four native rhizobial strains of PGP can be used as bio fertilisers as well as a bio control agent for enhancing the yield of blackgram in rice fallows.
- Subjects
RICE soils; RHIZOBIACEAE; PLANT growth promoting substances; FERTILIZERS; SOIL microbiology
- Publication
Malaysian Journal of Soil Science, 2021, Vol 25, p123
- ISSN
1394-7990
- Publication type
Article