We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Overview of Anti-SARS-CoV-2 Immune Response Six Months after BNT162b2 mRNA Vaccine.
- Authors
Gandolfo, Claudia; Anichini, Gabriele; Mugnaini, Marco; Bocchia, Monica; Terrosi, Chiara; Sicuranza, Anna; Gori Savellini, Gianni; Gozzetti, Alessandro; Franchi, Federico; Cusi, Maria Grazia
- Abstract
Background: We have designed a prospective study aiming to monitor the immune response in 178 health care workers six months after BNT162b2 mRNA vaccination. Methods: The humoral immune response of all subjects was evaluated by chemiluminescence (CMIA); in 60 serum samples, a live virus-based neutralization assay was also tested. Moreover, 6 months after vaccination, B- and T-cell subsets from 20 subjects were observed by FACS analysis after restimulation with the trimeric SARS-CoV-2 Spike protein as an antigen, thus mimicking reinfection in vitro. Results: A significant decrease of circulating IgG levels and neutralizing antibodies over time were observed. Moreover, six months after vaccination, a variable T-cell immune response after in vitro antigen stimulation of PBMC was observed. On the contrary, the analysis of B-cell response showed a shift from unswitched to switched memory B-cells and an increase of Th17 cells. Conclusions: Although the variability of the CD4+ and CD8+ immune response and an antibody decline was observed among vaccinated subjects, the increase of switched memory B-cells and Th17 cells, correlating with the presence of neutralizing antibodies, opened the debate on the correct timing of vaccination.
- Subjects
MEDICAL personnel; IMMUNE response; HUMORAL immunity; COVID-19 vaccines; T helper cells
- Publication
Vaccines, 2022, Vol 10, Issue 2, p171
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines10020171