We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Circ_0009910 regulates growth and metastasis and is associated with poor prognosis in gastric cancer.
- Authors
LIU, M.; CAI, J.; YAO, H.-W.; ZHANG, Z.-T.; LIU, K.-D.; ZHANG, L.; BAI, Y.-K.
- Abstract
OBJECTIVE: Circular RNAs (circRNAs) have recently shown capabilities as gene regulators in mammals. In this study, we aimed to evaluate the effects and mechanism of circ_0009910 in gastric cancer (GC). PATIENTS AND METHODS: Circ_0009910 expression was quantified by Real-time PCR in human GC cell lines and tissues. Association between circ_0009910 levels and clinicopathological factors and patient’s prognosis was analyzed. The roles of circ_0009910 in regulating GC cell proliferation, colony formation, migration, and invasion were evaluated in vitro. Western blot analysis was conducted to detect the expressions of molecular markers of epithelial- mesenchymal transition (EMT). RESULTS: Circ_0009910 expression level was elevated in GC tissues and cell lines and associated with clinical stage (p = 0.032), distant metastasis (p = 0.028) and differentiation (p = 0.007). Kaplan-Meier survival analysis indicated that circ_0009910 expression in positive group has a worse overall survival compared to the negative group (p = 0.0013). Multivariate analysis showed that circ_0009910 was an independent risk factor for GC (HR = 2.346, 95% CI: 1.673-3.775, p = 0.006). Knockdown of circ_0009910 expression can suppress BGC823 and AGS cells proliferation, migration and invasion in vitro experiments. The results of Western blot indicated that knockdown of circ_0009910 increased expression of E-cadherin and decreased expression of the mesenchymal markers, snail and N-cadherin. CONCLUSIONS: Altogether, we demonstrate that circ_0009910 acts as a prognostic biomarker and promote cell proliferation, migration, invasion and EMT in GC, indicating that circ_0009910 may be a novel potential biomarker and therapeutic target of GC.
- Subjects
GASTRIC diseases; METASTASIS; PROGNOSIS; NEOPLASTIC cell transformation; MESENCHYMAL stem cells
- Publication
European Review for Medical & Pharmacological Sciences, 2018, Vol 22, Issue 23, p8248
- ISSN
1128-3602
- Publication type
Article