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- Title
Metabotypes of Pseudomonas aeruginosa Correlate with Antibiotic Resistance, Virulence and Clinical Outcome in Cystic Fibrosis Chronic Infections.
- Authors
Moyne, Oriane; Castelli, Florence; Bicout, Dominique J.; Boccard, Julien; Camara, Boubou; Cournoyer, Benoit; Faudry, Eric; Terrier, Samuel; Hannani, Dalil; Huot-Marchand, Sarah; Léger, Claire; Maurin, Max; Ngo, Tuan-Dung; Plazy, Caroline; Quinn, Robert A.; Attree, Ina; Fenaille, François; Toussaint, Bertrand; Le Gouëllec, Audrey
- Abstract
Pseudomonas aeruginosa (P.a) is one of the most critical antibiotic resistant bacteria in the world and is the most prevalent pathogen in cystic fibrosis (CF), causing chronic lung infections that are considered one of the major causes of mortality in CF patients. Although several studies have contributed to understanding P.a within-host adaptive evolution at a genomic level, it is still difficult to establish direct relationships between the observed mutations, expression of clinically relevant phenotypes, and clinical outcomes. Here, we performed a comparative untargeted LC/HRMS-based metabolomics analysis of sequential isolates from chronically infected CF patients to obtain a functional view of P.a adaptation. Metabolic profiles were integrated with expression of bacterial phenotypes and clinical measurements following multiscale analysis methods. Our results highlighted significant associations between P.a "metabotypes", expression of antibiotic resistance and virulence phenotypes, and frequency of clinical exacerbations, thus identifying promising biomarkers and therapeutic targets for difficult-to-treat P.a infections
- Subjects
PSEUDOMONAS aeruginosa infections; BURKHOLDERIA infections; CYSTIC fibrosis; PSEUDOMONAS aeruginosa; TREATMENT effectiveness; DRUG resistance in bacteria; LUNG infections; SEQUENTIAL analysis
- Publication
Metabolites (2218-1989), 2021, Vol 11, Issue 2, p63
- ISSN
2218-1989
- Publication type
Article
- DOI
10.3390/metabo11020063