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- Title
Involvement of GRP78 in inhibition of HBV secretion by Boehmeria nivea extract in human HepG2 2.2.15 cells.
- Authors
Huang, K.-L.; Lai, Y.-K.; Lin, C.-C.; Chang, J.-M.
- Abstract
Previous studies showed that the root extract of Boehmeria nivea (BNE) can significantly suppress the production of hepatitis B virus (HBV) in vitro and in vivo. In this study, viral core and large-surface proteins accompanied with their encapsidated viral DNA were observed to accumulate within the cells. Notably, 78-kDa glucose-regulated protein (GRP78) was found to be suppressed by BNE, and stimulation of the GRP78 expression by thapsigargin could rescue virus production initially inhibited by BNE. The antiviral effect of BNE was reversible, which also coincided with the level of GRP78. Furthermore, we synthesized the GRP78 siRNA to knockdown the expression of GRP78 protein, and the production of supernatant HBV DNA was reduced simultaneously. Moreover, combined treatment of BNE and 3TC exhibited an additive anti-hepatitis B virus effect. In conclusion, the inhibitory effect of BNE on blocking assembled virion secretion might be via the reduction of GRP78.
- Subjects
HEPATITIS B treatment; BOEHMERIA; HERBAL medicine; SMALL interfering RNA; ENDOPLASMIC reticulum
- Publication
Journal of Viral Hepatitis, 2009, Vol 16, Issue 5, p367
- ISSN
1352-0504
- Publication type
Article
- DOI
10.1111/j.1365-2893.2009.01072.x