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- Title
Protein–protein interactions of tandem affinity purification-tagged protein kinases in rice.
- Authors
Rohila, Jai S.; Mei Chen; Shuo Chen; Chen, Johann; Cerny, Ronald; Dardick, Chris; Canlas, Patrick; Xia Xu; Gribskov, Michael; Kanrar, Siddhartha; Jian-Kang Zhu; Ronald, Pamela; Fromm, Michael E.
- Abstract
Forty-one rice cDNAs encoding protein kinases were fused to the tandem affinity purification (TAP) tag and expressed in transgenic rice plants. The TAP-tagged kinases and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by mass spectrometry. Ninety-five percent of the TAP-tagged kinases were recovered. Fifty-six percent of the TAP-tagged kinases were found to interact with other rice proteins. A number of these interactions were consistent with known protein complexes found in other species, validating the TAP-tag method in rice plants. Phosphorylation sites were identified on four of the kinases that interacted with either 14-3-3 proteins or cyclins.
- Subjects
PROTEIN-protein interactions; PROTEIN kinases; RICE; PROTEOMICS; PLANT proteins; PLANT cells &; tissues
- Publication
Plant Journal, 2006, Vol 46, Issue 1, p1
- ISSN
0960-7412
- Publication type
Article
- DOI
10.1111/j.1365-313X.2006.02671.x