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- Title
Identification of molecular markers linked to Rdr1, a gene conferring resistance to blackspot in roses.
- Authors
von Malek, B.; Weber, W. E; Debener, T.
- Abstract
Blackspot resistance in the tetraploid rose genotype 91/100–5 had been characterised previously as a single dominant gene in duplex configuration. In the present study a tetraploid progeny (95/3) segregating for the presence of the blackspot resistance gene Rdr1 were screened with 868 RAPD and 114 AFLP primers/primer combinations. Seven AFLP markers were found to be linked to Rdr1 at distances between 1.1 and 7.6 cM. The most closely linked AFLP marker was cloned and converted into a SCAR marker that could be screened in a larger population than the original AFLP and was linked at a distance of 0.76 cM. The cloned fragment was used as an RFLP probe to locate the marker on a chromosome map of diploid roses. This is the first report of markers linked to a resistance gene in roses, and the possibilities of using them for a marker-assisted selection for blackspot resistance as well as for map-based cloning approaches are discussed.
- Subjects
ROSE diseases &; pests; GENETIC markers; PLANT chromosomes; DISEASE resistance of plants; PLANT genetics; CLONING
- Publication
Theoretical & Applied Genetics, 2000, Vol 101, Issue 5/6, p977
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s001220051570