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- Title
Biotransformation of eugenol by suspension cultures of transgenic crown galls of Panax quinquefolium and suspension cultures of Nicotiana tabacum.
- Authors
Lin Yang; Chunyan Yan; Jianhua Zhu; Liyan Song; Rongmin Yu
- Abstract
The biocatalytic ability of transgenic crown galls of Panax quinquefolium was evaluated by using eugenol ( 1) as a substrate and suspension cultures of Nicotiana tabacum as control system. Three biotransformed products, namely: 2-methoxy-4-(2-propenyl)phenyl- O- β- d-glucopyranoside ( 2, 67.11%), 2-methoxy-4-(2-propenyl)phenyl- O- β- d-glucopyranosyl (6′ → 1″)- β- d-xylopyranoside ( 3, 2.85%) and methyl eugenol ( 4, 14.30%) were obtained after 5 days of administration of eugenol to the suspension cultures of transgenic crown galls of P. quinquefolium. In contrast, only one product, compound 2 (15.41%), was obtained in suspension cultures of N. tabacum after 5 days of incubation . The results indicated that the glycosylation ability of transgenic crown galls of P. quinquefolium was much higher than that of the cultured cells of N. tabacum.
- Subjects
ENZYMES; TRANSGENIC organisms; GALLS (Botany); AMERICAN ginseng; EUGENOL; TOBACCO
- Publication
World Journal of Microbiology & Biotechnology, 2010, Vol 26, Issue 7, p1201
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-009-0289-7