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- Title
Influence of As2O3 combined with ginsenosides Rg3 on inhibition of lung cancer NCI-H1299 cells and on subsistence of nude mice bearing hepatoma.
- Authors
Che, Jian-Bo; Liu, Zhong-Hua; Ma, Hong-Bing; Li, Yong; Zhao, Hui; Li, Xiao-Hui; Liu, Wei-Chao; Shi, Gong-Ning
- Abstract
Objectives To study the effect of arsenic trioxide (As 2 O 3 ) combined with ginsenosides Rg3 on inhibiting the NCI-H1299 lung cancer cells and subsistence in nude mice bearing hepatoma. Methods MTT method was used to measure the inhibition effect of As 2 O 3 combined Rg3 on NCI-H1299 cells, and the proliferation inhibiting effect was observed via establishing the transplanted tumor model in vitro . A total of 40 tumor-bearing nude mice were randomly divided into normal saline group, As 2 O 3 , Rg3 and As 2 O 3 +Rg3 group. Transplantation tumor model of lung cancer in nude mice was constructed, followed by injection of certain concentrations of normal saline, As 2 O 3 , ginseng saponin Rg3 and As 2 O 3 +Rg3 every day. The survival duration and the tumors size of the mice were recorded and the Kaplan-Meier curve was made; microscopic observation of apoptosis of tumor cells in vivo was done using TUNEL staining. Results After 72 h of injection, inhibition rate of tumor cell in normal saline group, As 2 O 3 group, Rg3 group and As 2 O 3 +Rg3 group was (5.66±0.31)%, (65.58±4.75)%, (44.69±3.32)% and (82.67±5.43)%, respectively. Inhibition rate of tumor cell in As 2 O 3 group, Rg3 group and As 2 O 3 +Rg3 group was significantly higher than that of normal saline group ( P <0.01); inhibition rate of tumor cells of As 2 O 3 +Rg3 group was significantly higher than that of the two groups given As 2 O 3 or Rg3 alone ( P <0.01). The tumor volume of As 2 O 3 group, Rg3 group and As 2 O 3 +Rg3 group shrank to (65.38±3.25)%, (77.68±3.43)% and (42.65±3.55)% of the original, tumor volume of saline group was 1.21 times of the original size ( P <0.01); Median survival of saline group, Rg3 group, As 2 O 3 group were significantly shorter than that of As 2 O 3 +Rg3 group ( P <0.01); co-ordinated intervention ability of As 2 O 3 +Rg3 on NCI-H1299 cell was significantly higher than that of As 2 O 3 or Rg3, separately. Conclusions As 2 O 3 combined with Rg3 can significantly inhibit proliferation of NCI-H1299 cells in lung cancer, prolong survival of tumor-bearing nude mice, and promote tumor cell apoptosis, and have significant effect on lung cancer treatment.
- Publication
Asian Pacific Journal of Tropical Medicine, 2014, Vol 7, Issue 10, p772
- ISSN
1995-7645
- Publication type
Article
- DOI
10.1016/S1995-7645(14)60134-6