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- Title
A novel curcumin derivative CL-6 exerts antitumor effect in human gastric cancer cells by inducing apoptosis through Hippo–YAP signaling pathway.
- Authors
Ye, Chenmin; Wang, Wenqian; Xia, Guojun; Yu, Chengyang; Yi, Yongdong; Hua, Chunyan; Tu, Fuyang; Shen, Leibin; Chen, Canjin; Sun, Weijian; Zheng, Zhiqiang
- Abstract
Purpose: Gastric carcinoma is the second most frequently diagnosed cancer and leading cause of cancer death in China. As a new generation of cancer therapeutic drug, CL-6, a curcumin derivative, shows better bioavailability than curcumin, which has shown anticancer effects in gastric cancer (GC). However, whether CL-6 shows similar activities in GC has not been examined. Materials and methods: Cell proliferation assay, colony-forming assay, flow cytometric analysis, wound healing assay, and Transwell invasion assay were performed to examine the effects of CL-6 on proliferation, apoptosis, migration, and invasion on human AGS and MGC-803 cell lines. Western blot was used to evaluate protein levels of Bax, Bcl-2, YAP, p-YAP, and Lats, and gene expression was measured by real-time quantitative PCR (RT-qPCR). Results: CL-6 dose dependently reduced proliferation, increased apoptosis, and inhibited the migration and invasion abilities of AGS and MGC-803 cells. CL-6 also increased levels of pro-apoptotic protein Bax, decreased levels of antiapoptotic protein Bcl-2, and increased the Bax/Bcl-2 ratio. CL-6 treatment also inhibited YAP and YAP protein and mRNA expression, while it induced the expression of Lats and p-YAP (Ser127). Conclusion: CL-6 induces apoptosis of GC cells by activating the Hippo–YAP signaling pathway. These results indicate the therapeutic potential of the novel curcumin derivative CL-6 in GC.
- Subjects
CHINA; CURCUMINOIDS; STOMACH cancer; CANCER cells; BAX protein; BCL-2 proteins; CURCUMIN
- Publication
OncoTargets & Therapy, 2019, Vol 12, p2259
- ISSN
1178-6930
- Publication type
Article
- DOI
10.2147/OTT.S196914