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- Title
The conclusiveness of less-invasive imaging techniques (computer tomography, X-ray) with regard to their identification of bone diseases in a primate model ( Callithrix jacchus).
- Authors
Grohmann, J.; Taetzner, S.; Theuss, T.; Kuehnel, F.; Buchwald, U.; Einspanier, A.
- Abstract
Background Although common marmosets seem to be appropriate animal models to examine bone diseases, no data about the conclusiveness of less-invasive techniques are available. Therefore, the aim was to combine different techniques to analyse changes in bone metabolism of common marmosets with bone diseases. Methods Five monkeys were examined by X-ray, computer tomography (CT), histology and immunohistochemistry (IHC). Results Monkeys with lowest bone mineral density (BMD) showed increased bone marrow, decreased cancellous bone and decreased contrast in X-ray. Highest alkaline phosphatase (AP)-levels were detected in bones with low elastic modulus. Expression of osteopontin (OPN), osteocalcin (OC) and runt-related transcriptions factor 2 (RUNX 2) was detected in bones with high modulus. No expression was present in bones with lower modulus. Collagen type I and V were found in every bone. Conclusions In conclusion, CT, X-ray and AP are useful techniques to detect bone diseases in common marmosets. These observations could be confirmed by IHC.
- Subjects
DIAGNOSIS of bone diseases; ANIMAL disease models; TOMOGRAPHY; X-rays; MEDICAL imaging systems; ANIMAL models in research; IMMUNOHISTOCHEMISTRY; PRIMATES as laboratory animals
- Publication
Journal of Medical Primatology, 2012, Vol 41, Issue 2, p130
- ISSN
0047-2565
- Publication type
Article
- DOI
10.1111/j.1600-0684.2011.00531.x