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- Title
Quantification of vitellin/vitellogenin-like proteins in the oyster Crassostrea corteziensis (Hertlein 1951) as a tool to predict the degree of gonad maturity.
- Authors
Arcos, Fabiola G.; Ibarra, Ana María; del Carmen Rodríguez-Jaramillo, María; García-Latorre, Ethel Awilda; Vazquez-Boucard, Celia
- Abstract
The oyster's reproductive process is poorly documented, especially in terms of a quantitative approach. In recent years, investigations with this species have been directed at determining important reproductive factors. Within this scope, techniques that allow standardized and accurate quantitative estimations of gonad development have become of primary importance. In this study, the histological characteristics and the levels of vitellin/vitellogenin-like proteins (Vn/Vtg) from ovaries of the Mexican Pacific ‘pleasure’ oyster Crassostrea corteziensis (Hertlein 1951) were analysed during different stages of gonad maturation using quantitative histological techniques and an enzyme-linked immunosorbent assay. This was performed in order to evaluate a possible quantitative tool to predict the degrees of gonad maturity and to analyse the biological implications of the findings relative not only to broodstock conditioning but also to natural populations. Using this information, we expect to be able to undertake further research on different reproductive aspects of this oyster species, including, among others, evaluation of the response in Vn/Vtg concentrations to different diets and environmental conditions during laboratory conditioning.
- Subjects
CRASSOSTREA; OYSTER fisheries; OYSTERS; GONAD physiology; FISH histology; BROOD stock assessment
- Publication
Aquaculture Research, 2009, Vol 40, Issue 6, p644
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/j.1365-2109.2008.02138.x