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- Title
Contradictory results in pollen viability determination of Valeriana scandens L.
- Authors
Duarte-Silva, Erica; Rodrigues, Lia R.; Mariath, Jorge E.A.
- Abstract
Methods for estimating pollen viability may offer contradictory inferences. Based on this, we investigated the pollen viability from the perfect flowers of Valeriana scandens based on three methods: (1) Alexander stain, (2) FDA - fluorescein diacetate and (3) in vitro pollen germination. As results, V. scandens pollen viability was 95% with Alexander, 47% with FDA reaction and 30% with pollen germination. Analysis of variance indicated that the three techniques offer distinct inferences and it was not possible to estimate pollen germination performing Alexander or FDA reaction. A complementary test with propionic-carmine was performed and showed 98% of pollen stainability. Considering that in vitro pollen germination is the quantitative method that presents the highest correlation with seed set, Alexander and carmine tests overestimated the pollen viability in V. scandens. We conclude that stainability tests were inconsistent to measure pollen viability and estimate pollen germination.
- Subjects
POLLEN; VALERIANA; FLUORESCEIN; GERMINATION; ANALYSIS of variance
- Publication
Gene Conserve, 2011, Vol 10, Issue 41, p234
- ISSN
1808-1878
- Publication type
Article