We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Salivary Levels of NLRP3 Inflammasome-Related Proteins as Potential Biomarkers of Periodontal Clinical Status.
- Authors
Isaza‐Guzmán, Diana M.; Medina‐Piedrahíta, Verónica M.; Gutiérrez‐Henao, Carolina; Tobón‐Arroyave, Sergio I.; Isaza-Guzmán, Diana M; Medina-Piedrahíta, Verónica M; Gutiérrez-Henao, Carolina; Tobón-Arroyave, Sergio I
- Abstract
<bold>Background: </bold>Emerging evidence suggests that activation of inflammasomes plays a central mechanism in pathogenesis of periodontitis. This study aims to compare salivary levels of nod-like receptor family pyrin domain containing protein (NLRP) 3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), cysteine aspartase (caspase)-1, and interleukin (IL)-1β from individuals with aggressive (AgP) or chronic periodontitis (CP) and healthy controls (HC), as well as elucidate its association with periodontal clinical status.<bold>Methods: </bold>Saliva samples from individuals with CP (n = 75), AgP (n = 20), and HC (n = 69) were collected. Periodontal status was assessed by measurement of probing depth, clinical attachment level, and extent and severity of disease. Salivary levels of analytes were analyzed by enzyme-linked immunosorbent assay. Association between biomarkers with CP or AgP was analyzed using multivariate binary logistic regression models.<bold>Results: </bold>Significantly higher levels of NLRP3, ASC, and IL-1β were detected in periodontitis groups in comparison to the periodontally HC group. However, no significant differences were observed for caspase-1 levels between clinical groups, and only NLRP3 salivary concentration was significantly higher in AgP compared with CP patients. Also, positive significant correlations among NLRP3, ASC, and IL-1β salivary concentrations and clinical parameters were observed. Logistic regression analyses revealed a strong/independent association of NLRP3, ASC, and IL-1β salivary levels with CP and AgP.<bold>Conclusion: </bold>Although the concentration of caspase-1 in saliva samples makes its determination useless for detection of periodontal disease and/or its severity, salivary levels of NLRP3, ASC, and IL-1β may act as strong/independent indicators of amount and extent of periodontal breakdown in both CP and AgP and could potentially be used for prevention and therapy of this group of diseases.
- Subjects
SALIVA analysis; PYRIN (Protein); INFLAMMASOMES; PERIODONTAL disease; BIOMARKERS; AGGRESSIVE periodontitis; APOPTOSIS; PROTEINS in the body; PATIENTS; CHRONIC disease diagnosis; PROTEIN analysis; CHRONIC diseases; ENZYME-linked immunosorbent assay; PERIODONTITIS; PROTEOLYTIC enzymes; SALIVA; CASE-control method; DIAGNOSIS
- Publication
Journal of Periodontology, 2017, Vol 88, Issue 12, p1329
- ISSN
0022-3492
- Publication type
journal article
- DOI
10.1902/jop.2017.170244