We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Introduction of a Mixture of β-Tricalcium Phosphate Into a Complex of Bone Marrow Mesenchymal Stem Cells and Type I Collagen Can Augment the Volume of Alveolar Bone Without Impairing Cementum Regeneration.
- Authors
Nagahara, Takayosi; Yoshimatsu, Shinichiro; Shiba, Hideki; Kawaguchi, Hiroyuki; Takeda, Katsuhiro; Iwata, Tomoyuki; Mizuno, Noriyoshi; Fujita, Tsuyoshi; Kurihara, Hidemi
- Abstract
Background: The purpose of this study is to evaluate whether (β-tricalcium phosphate (β-TCP) could be a promising modality to help augment alveolar bone in periodontal tissue regeneration by bone marrow mesenchymal stem cells (BMMSCs). Methods: Expanded BMMSCs and atelocollagen (Col) were mixed together (MSC/Col). A combination of β-TCP with MSC/Col was also prepared (MSC/Col/TCP). MSC/ Col/TCP or MSC/Col was transplanted into experimental periodontal Class III furcation defects that had been exposed to inflammation in beagle dogs. Periodontal tissue regeneration was evaluated by histologic and morphometric analyses at 4 and 8 weeks after transplantation. Results: MSC/Col and MSC/Col/TCP enhanced periodontal tissue regeneration compared to Col and TCP/Col according to hematoxylin and eosin staining. The percentage of new cementum length in the MSC/Col/TCP group was not significantly different from that in the MSC/Col group at 4 and 8 weeks. On the other hand, the percentage of new bone area in the MSC/Col/TCP group was much higher than that in the MSC/TCP group at 4 weeks. However, at 8 weeks, no significant difference in new bone area was found between the two groups. In the MSC/Col/TCP group, β-TCP was surrounded by newly formed bone. Multinucleated cells, which were positive for osteopontin and tartrate-resistant acid phosphatase, were present in the interconnected mac-ropores of β-TCP. Conclusion: These findings suggest that β-TCP is applicable as a scaffold for BMMSCs transplantation and helps augment alveolar bone without impairing regeneration of cementum.
- Subjects
CALCIUM phosphate; GUIDED tissue regeneration; MESENCHYMAL stem cells; TOOTH socket; ALVEOLAR process; PERIODONTAL ligament; CELL transplantation; CEMENTUM; THERAPEUTICS
- Publication
Journal of Periodontology, 2015, Vol 86, Issue 3, p456
- ISSN
0022-3492
- Publication type
Article
- DOI
10.1902/jop.2014.140384