We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Preparation and Enantioseparation of a New Click Derived β-Cyclodextrin Chiral Stationary Phase.
- Authors
Fan, Qing; Zhang, Kui; Tian, Li-wen; Fan, Jun; Zheng, Sheng-run; Zhang, Wei-Guang
- Abstract
A new cyclodextrin-derived chiral stationary phase (denoted as CDA-CSP) was synthesized by immobilizing mono(6A-azido-6A-deoxy)-per(p-chlorophenyl carbamoylated) β-cyclodextrin derivative to alkynyl modified silica via click chemistry. This newly prepared CSP shows good enantioseparation performance for six chiral compounds (1–6), such as 4-phenyl-oxazolidine-2-thione, two kinds of aryl alcohols, substituted flavonoids and benzoin, in which baseline separation of Analytes 1–4 was achieved under the experimental conditions. The effects of column temperature, mobile phase pH and content of methanol on the enantioseparation characteristics of CDA-CSP were investigated in detail. Retention factor and resolution for Compound 3 gradually reduced with an increase of column temperature, and a good linear relationship was shown between napierian logarithm of selectivity factor and reciprocal of column temperature. In the pH range from 3.56 to 5.50, a change in pH hardly affected the resolution of Analyte 2. In addition, increasing methanol in the mobile phase resulted in rapid eluting of the analytes from the column in reversed-phase mode. The retention factors for Analytes 1 and 3 significantly decreased and their resolution showed different trends.
- Subjects
CYCLODEXTRINS; CHIRAL stationary phases; FLAVONOIDS; HIGH performance liquid chromatography; NUCLEAR magnetic resonance
- Publication
Journal of Chromatographic Science, 2014, Vol 52, Issue 5, p453
- ISSN
0021-9665
- Publication type
Article
- DOI
10.1093/chromsci/bmt060