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- Title
The protective role of yeast Cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p)
- Authors
Pereira, Helena; Azevedo, Flávio; Rego, António; Sousa, Maria João; Chaves, Susana R.; Côrte-Real, Manuela
- Abstract
Abstract: We have previously shown that the yeast Cathepsin D (CatD) Pep4p translocates from the vacuole to the cytosol during acetic acid-induced apoptosis and is required for efficient mitochondrial degradation, though its specific role in this process is still elusive. Here, we show that the protective role of Pep4p in acetic acid-induced apoptosis depends on its catalytic activity and is independent of the yeast voltage-dependent anion channel Por1p (which has no role on mitochondrial degradation) but dependent on AAC proteins, the yeast adenine nucleotide translocator. Our results demonstrate a differential interplay between yeast vacuolar CatD and mitochondrial proteins involved in apoptosis regulation.
- Subjects
CATHEPSIN D; ACETIC acid; APOPTOSIS inducing factor; REACTIVE oxygen species; PROPIDIUM iodide; CYTOSOL; ADENINE nucleotide translocase; ADENOSINE triphosphate
- Publication
FEBS Letters, 2013, Vol 587, Issue 2, p200
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.11.025