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- Title
Cell cycle regulation of the mammalian CDK activator RINGO/Speedy A
- Authors
Dinarina, Ana; Santamaria, Patricia G.; Nebreda, Angel R.
- Abstract
Abstract: Cell cycle progression is regulated by cyclin-dependent kinases (CDKs), whose activation requires the binding of regulatory subunits named cyclins. RINGO/Speedy A is a mammalian protein that has no amino acid sequence homology with cyclins but can activate CDKs. Here we show that RINGO/Speedy A is a highly unstable protein whose expression and phosphorylation are periodically regulated during the cell cycle. RINGO/Speedy A is degraded by the proteasome and the process involves the ubiquitin ligase SCFSkp2. Overexpression of a stabilized RINGO/Speedy A form results in the accumulation of high levels of RINGO/Speedy A at late stages of mitosis, which interfere with cytokinesis and chromosome decondensation. Our data show that tight regulation of RINGO/Speedy A is important for the somatic cell cycle. Structured summary: MINT-7226413:RINGO A (uniprotkb:Q5MJ70) physically interacts (MI:0914) with Ubiquitin (uniprotkb:P62988) by anti bait coimmunoprecipitation (MI:0006)MINT-7226431, MINT-7226448:RINGO A (uniprotkb:Q5MJ70) physically interacts (MI:0914) with Skp2 (uniprotkb:Q13309) by anti tag coimmunoprecipitation (MI:0007)
- Subjects
CELL cycle regulation; CYCLIN-dependent kinases; MAMMAL cytology; PROTEIN binding; CYCLINS; AMINO acid sequence; UBIQUITIN; GENE expression
- Publication
FEBS Letters, 2009, Vol 583, Issue 17, p2772
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.07.028