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- Title
SHIP2 associates with intersectin and recruits it to the plasma membrane in response to EGF
- Authors
Xie, Jingwei; Vandenbroere, Isabelle; Pirson, Isabelle
- Abstract
Abstract: We identified intersectin1 (ITSN1) as a new binding partner of the SH2 domain containing inositol 5-phosphatase 2 (SHIP2). The interaction between SHIP2 and ITSN1 was confirmed in vivo. Src homology 3D, A, C, and E domains of ITSN1 were shown to be implicated in the interaction. In response to epidermal growth factor, SHIP2 expression could recruit the ITSN1 short form (ITSN1-S) to the cell membrane, while SHIP2 overexpression did not modulate the ITSN-mediated extracellular signal-regulated kinase1/2 and c-Jun NH2-terminal kinase activation. Our data provide a molecular link between SHIP2 and ITSN1 which are involved in receptor endocytosis regulation. Structured summary: MINT-6673604: ITSN-1S (uniprotkb:Q15811-2) and SHIP2 (uniprotkb:O15357) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-6673646, MINT-6694843: SHIP2 (uniprotkb:Q6P549) physically interacts (MI:0218) with ITSN-1S (uniprotkb:Q9Z0R4-2) by anti bait coimmunoprecipitation (MI:0006) MINT-6673386: SHIP2 (uniprotkb:O15357) physically interacts (MI:0218) with ITSN-1S (uniprotkb:Q15811-2) by two hybrid (MI:0018) MINT-6673429, MINT-6673447, MINT-6673475: ITSN-1S (uniprotkb:Q15811-2) physically interacts (MI:0218) with SHIP2 (uniprotkb:O15357) by anti tag coimmunoprecipitation (MI:0007) MINT-6673513, MINT-6673542, MINT-6673557,MINT-6673572: ITSN-1S (uniprotkb:Q15811-2) physically interacts (MI:0218) with SHIP2 (uniprotkb:O15357) by pull down (MI:0096) MINT-6673462: ITSN-1L (uniprotkb:Q15811-1) physically interacts (MI:0218) with SHIP2 (uniprotkb:O15357) by anti tag coimmunoprecipitation (MI:0007)
- Subjects
INOSITOL phosphates; EPIDERMAL growth factor; CELL membranes; ENDOCYTOSIS; FLUORESCENCE microscopy
- Publication
FEBS Letters, 2008, Vol 582, Issue 20, p3011
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.07.048