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- Title
Osteoactivin fragments produced by ectodomain shedding induce MMP-3 expression via ERK pathway in mouse NIH-3T3 fibroblasts
- Authors
Furochi, Harumi; Tamura, Seiko; Mameoka, Mai; Yamada, Chiharu; Ogawa, Takayuki; Hirasaka, Katsuya; Okumura, Yuushi; Imagawa, Takahito; Oguri, Sachiko; Ishidoh, Kazumi; Kishi, Kyoichi; Higashiyama, Shigeki; Nikawa, Takeshi
- Abstract
Abstract: Intact osteoactivin, a novel type I membrane glycoprotein, were shed at a dibasic motif in the juxtamembrane region in C2C12 myoblasts. Extracellular fragments were secreted into the culture media by a putative metalloprotease. Extracellular fragments of osteoactivin, but not control protein, induced matrix metalloprotease-3 (MMP-3) expression in NIH-3T3 fibroblasts. Epidermal growth factor (ERK) kinase inhibitors inhibited the osteoactivin-mediated MMP-3 expression, whereas the extracellular fragment of osteoactivin activated ERK1/2 and p38 in the mitogen-activated protein kinase pathway. Our results suggest that the extracellular fragments of osteoactivin produced by shedding act as a growth factor to induce MMP-3 expression via the ERK pathway in fibroblasts.
- Subjects
GLYCOPROTEINS; METALLOPROTEINASES; FIBROBLASTS; EPIDERMAL growth factor
- Publication
FEBS Letters, 2007, Vol 581, Issue 30, p5743
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.036