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- Title
Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense
- Authors
Germain, Hugo; Houde, Josée; Gray-Mitsumune, Madoka; Sawasaki, Tatsuya; Endo, Yaeta; Rivoal, Jean; Matton, Daniel P.
- Abstract
Abstract: Solanum chacoense ovule receptor kinase 28 (ScORK28) was found among 30 receptor kinases from an ovule cDNA library enriched for weakly expressed mRNAs. This LRR-RLK displayed high level of tissue specificity at the RNA and protein levels and was predominantly expressed in female reproductive tissues. Protein expression analyses in planta revealed that ScORK28 was N-glycosylated and ScORK28::GFP fusion analyses showed that it was localized at the plasma membrane. Bacterial expression of ScORK28 catalytic domain followed by kinase activity assays revealed that ScORK28 is an active Mg2+-dependent protein kinase and that the juxtamembrane domain is necessary for kinase activity.
- Subjects
PROTEIN kinases; RNA; PROTEINS; TISSUES
- Publication
FEBS Letters, 2007, Vol 581, Issue 26, p5137
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.10.001