We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Post-conditioning with cyclosporine A fails to reduce the infarct size in an in vivo porcine model.
- Authors
LIE, R. H.; STOETTRUP, N.; SLOTH, E.; HASENKAM, J. M.; KROYER, R.; NIELSEN, T. T.
- Abstract
Background: Cyclosporine A has generated intense interest in the field of cardioprotection due to its ability to protect the mitochondria at reperfusion by blocking the opening of the mitochondrial permeability transition pore. The aim of our study was to examine the cardioprotective effect of Sandimmun®, a clinically available formulation of cyclosporine A, in an in vivo large mammal model. Methods: Forty-eight pigs were randomly allocated to one of three groups: (i) Control group (Con, n=19), (ii) Cyclosporine group, (Cyclo, n=19) Sandimmun® 10 mg/kg i.v. bolus 5 min before reperfusion and (iii) Pre-conditioning group (Precon, n=10) two cycles of 10 min ischemia interspersed with 30-min reperfusion. The study was further sub-divided into a metabolic protocol, evaluating myocardial metabolism by measuring changes in the interstitial lactate concentration, and a coronary flow protocol. All animals were subjected to 40 min of left anterior descending coronary artery occlusion, followed by 180 min of reperfusion before histochemical staining and assessment of infarct size by planimetry. Results: Infarct sizes were measured as: Con 51.4 ± 16.5%, Cyclo 47.3 ± 15.7% and Precon 2.4 ± 3.6%, with no significant difference between the Con and Cyclo groups but a highly significant difference between the Precon and Cyclo and Con groups ( P<0.0001 for both comparisons). In the Cyclo group, the interstitial lactate concentration was significantly increased compared with the Con group at 6-min reperfusion, although significantly lower at 14 min presumably due to accelerated washout. Conclusion: In this large animal model, a 10 mg/kg bolus administration of Sandimmun® 5 min before reperfusion did not reduce the infarct size.
- Subjects
CYCLOSPORINE; IMMUNOSUPPRESSIVE agents; ISCHEMIA; CORONARY arteries; BLOOD circulation disorders
- Publication
Acta Anaesthesiologica Scandinavica, 2010, Vol 54, Issue 7, p804
- ISSN
0001-5172
- Publication type
Article
- DOI
10.1111/j.1399-6576.2010.02241.x