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- Title
MicroRNA-125b regulates proliferation and radioresistance of oral squamous cell carcinoma.
- Authors
Shiiba, M; Shinozuka, K; Saito, K; Fushimi, K; Kasamatsu, A; Ogawara, K; Uzawa, K; Ito, H; Takiguchi, Y; Tanzawa, H
- Abstract
Background:MicroRNAs (miRNAs) are involved in essential biological activities, and have been reported to exhibit differential expression profiles in various cancers. Our previous study demonstrated that intercellular adhesion molecule-2 (ICAM2) inhibition induces radiosensitisation in oral squamous cell carcinoma (OSCC) cells. Thus, we hypothesised that certain miRNAs play crucial roles in radioresistance in OSCC by regulating ICAM2 expression.Methods:Because predicted target gene analyses revealed that microRNA-125b (miR-125b) potentially regulates ICAM2 mRNA expression, we examined the association between miR-125b and radioresistance. The expression of miR-125b was investigated by real-time quantitative reverse transcriptase-PCR. For a functional analysis, miR-125b was transfected to OSCC-derived cells.Results:A downregulated expression of miR-125b was found in OSCC-derived cell lines and OSCC samples. The miR-125b-transfected cells showed a decreased proliferation rate, enhanced radiosensitivity to X-ray irradiation and diminished ICAM2 mRNA expression. Moreover, miR-125b expression correlated with OSCC tumour staging and survival.Conclusion:These findings suggested that the downregulated miR-125b expression was associated with proliferation and radioresistance mechanisms, probably through ICAM2 signalling. Thus, controlling the expression or activity of miR-125b might contribute to suppressing proliferation and overcoming radioresistance in OSCC.
- Subjects
NON-coding RNA; CELL proliferation; SQUAMOUS cell carcinoma; TREATMENT of oral cancer; CELL adhesion molecules; MESSENGER RNA; GENE expression
- Publication
British Journal of Cancer, 2013, Vol 108, Issue 9, p1817
- ISSN
0007-0920
- Publication type
Article
- DOI
10.1038/bjc.2013.175