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- Title
Intradermal Delivery of Recombinant Vaccinia Virus Vector DIs Induces Gut-Mucosal Immunity.
- Authors
Yoshino, N.; Kanekiyo, M.; Hagiwara, Y.; Okamura, T.; Someya, K.; Matsuo, K.; Ami, Y.; Sato, S.; Yamamoto, N.; Honda, M.
- Abstract
Antigen-specific mucosal immunity is generally induced by the stimulation of inductive mucosal sites. In this study, we found that the replication-deficient vaccinia virus vector, DIs, generates antigen-specific mucosal immunity and systemic responses. Following intradermal injection of recombinant DIs expressing simian immunodeficiency virus gag (rDIsSIV gag), we observed increased levels of SIV p27-specific IgA and IgG antibodies in faecal extracts and plasma samples, and antibody-forming cells in the intestinal mucosa and spleen of C57BL/6 mice. Antibodies against p27 were not detected in nasal washes, saliva, and vaginal washes. The enhanced mucosal and systemic immunity persisted for 1 year of observation. Induction of Gag-specific IFN-γ spot-forming CD8+ T cells in the spleen, small intestinal intraepithelial lymphocytes, and submandibular lymph nodes was observed in the intradermally injected mice. Heat-inactivated rDIsSIV gag rarely induced antigen-specific humoral and T-helper immunity. Moreover, rDIsSIV gag was detected in MHC class II IA antigen-positive (IA+) cells at the injection site. Consequently, intradermal delivery of rDIs effectively induces antigen-specific humoral and cellular immunity in gut-mucosal tissues of mice. Our data suggest that intradermal injection of an rDIs vaccine may be useful against mucosally transmitted pathogens.
- Subjects
IMMUNITY; PRESERVATION of organs, tissues, etc.; IMMUNOGLOBULINS; EXOCRINE secretions; LEUCOCYTES
- Publication
Scandinavian Journal of Immunology, 2010, Vol 72, Issue 2, p98
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2010.02416.x