We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differential Locomotion of Long- and Short-term IL-2-activated Murine Natural Killer Cells in a Model Matrix Environment.
- Authors
Albertsson, P.; Basse, P. H.; Edsparr, K.; Kim, M. H.; Golfar, R. H.; Kitson, R. P.; Lennernäs, B.; Nannmark, U.; Johansson, B. R.
- Abstract
Tumour infiltration by activated natural killer (A-NK) cells is a pre-requisite for tumour eradication by adoptive NK cell transfer. Extravasated A-NK cells do not always succeed in reaching the crucial target cell conjugation. Therefore, we wished to study A-NK cell locomotion and interactions with melanoma cells in a matrix environment (Matrigel) by electron, confocal and fluorescence microscopy. Two distinct patterns of A-NK cell-mediated matrix disintegration were revealed during incubation of tumour cells and A-NK cells in Matrigel: (1) A-NK cells pre-cultured for 5 days altered the homogeneous texture of the Matrigel, an initial microporous appearance became a loose filamentous meshwork by 24 h. Matrix degrading protease inhibitors could not fully prevent this, but could delay the process; and (2) A-NK cells pre-cultured for 6 days or more, instead formed large excavations in the Matrigel leaving the remaining matrix less affected compared to the effects by the younger A-NK cells. By histochemical staining with Cupromeronic Blue, the excavations were shown to contain proteoglycan material. Protease inhibitors had no discernable effect on the development of the excavations. The conspicuous capacity of A-NK cells to disintegrate extracellular matrix and the formation of large excavations seems only partially to depend on matrix-degrading proteases. Formation of extracellular proteoglycan material is suggested to facilitate A-NK cell locomotion within a matrix environment.
- Subjects
KILLER cells; CELL transformation; EXTRACELLULAR matrix; MELANOCYTES; PROTEASE inhibitors; FLUORESCENCE microscopy; CONFOCAL microscopy
- Publication
Scandinavian Journal of Immunology, 2007, Vol 66, Issue 4, p402
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2007.01956.x