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- Title
Down-regulation of Musashi-2 exerts antileukemic effects on acute lymphoblastic leukemia cells and increases sensitivity to dexamethasone.
- Authors
Zou, Duobing; Lv, Mei; Chen, Ying; Niu, Tingting; Ma, Chao; Shi, Cong; Huang, Zhenya; Wu, Ying; Yang, Shujun; Wang, Yun; Wu, Ningning; Zhang, Yi; Ouyang, Guifang; Mu, Qitian
- Abstract
Musashi-2 (MSI2), implicated in the oncogenesis and propagation of a broad array of malignancies, inclusive of certain leukemia, remains a nascent field of study within the context of acute lymphoblastic leukemia (ALL). Using lentiviral transfection, ALL cells with stable MSI2 knockdown were engineered. A suite of analytic techniques – a CCK-8 assay, flow cytometry, qRT-PCR, and western blotting – were employed to evaluate cellular proliferation, cell cycle arrest, and apoptosis and to confirm differential gene expression. The suppression of MSI2 expression yielded significant results: inhibition of cell proliferation, G0/G1 cell cycle arrest, and induced apoptosis in ALL cell lines. Furthermore, it was noted that MSI2 inhibition heightened the responsiveness of ALL cells to dexamethasone. Significantly, the depletion of MSI2 prompted the translocation of GR from the cytoplasm to the nucleus upon dexamethasone treatment, consequently leading to enhanced sensitivity. Additionally, the FOXO1/4 signaling pathway contributed to the biological effects of ALL cells evoked by MSI2 silencing. Our study offers novel insight into the inhibitory effects of MSI2 suppression on ALL cells, positing MSI2 as a promising therapeutic target in the treatment of ALL.
- Subjects
LYMPHOBLASTIC leukemia; ACUTE leukemia; CELL cycle; CELL proliferation; GENE expression; CYTARABINE
- Publication
Annals of Hematology, 2024, Vol 103, Issue 1, p141
- ISSN
0939-5555
- Publication type
Article
- DOI
10.1007/s00277-023-05468-z