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- Title
The amino acid and carnitine concentration changes in bronchoalveolar lavage fluid from lung cancer patients.
- Authors
Sun, Xiaojun; Xu, Mengmin; Ding, Liren; Yang, Guobiao; Kong, Jianlong; Zhu, Yafang; Chen, Xuefang; Xu, Liang; Shan, Yisha; Xu, Yupin
- Abstract
Objective: To screen out potential biomarkers by analyzing fundamental nutrients in the bronchoalveolar lavage fluid (BALF) before confirming the lung cancer. Methods: In this study, 44 patients were enrolled with clinical information. The concentrations of 23 amino acids and 35 carnitines in their BALF were detected with the high-performance liquid chromatography–mass spectrometry (HPLC–MS). Combined with clinicopathological diagnosis, the patients were divided into the lung cancer group (grades I & II and III & IV) and the non-cancer group for standard statistical analysis. Results: The partial least squares-discriminant analysis (PLS-DA), the Shapiro–Wilk test, and the Bonferroni correction results showed that the serine concentration was higher and the butane-diacyl-carnitine (C4DC) concentration was lower in the lung cancer group, further showing the same changing trend continuously through the non-cancer stage, grades I & II stage and grades III & IV stage. Those two potential biomarkers have been identified. Conclusion: The HPLC–MS target detection in clinic for nutrient concentration levels is a promising technique to find the changing concentration of serine and C4DC in BALF, which provides an economical and practical way for early warning of lung cancer.
- Subjects
LUNG cancer; AMINO acids; LIQUID chromatography-mass spectrometry; BRONCHOALVEOLAR lavage; CANCER patients
- Publication
World Journal of Surgical Oncology, 2022, Vol 20, Issue 1, p1
- ISSN
1477-7819
- Publication type
Article
- DOI
10.1186/s12957-022-02850-5