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- Title
Extraction and Yield Optimisation of Fucose, Glucans and Associated Antioxidant Activities from Laminaria digitata by Applying Response Surface Methodology to High Intensity Ultrasound-Assisted Extraction.
- Authors
Garcia-Vaquero, Marco; Sweeney, Torres; Rajauria, Gaurav; O’Doherty, John; Tiwari, Brijesh
- Abstract
The objectives of this study were to employ response surface methodology (RSM) to investigate and optimize the effect of ultrasound-assisted extraction (UAE) variables, temperature, time and amplitude on the yields of polysaccharides (fucose and total glucans) and antioxidant activities (ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from <italic>Laminaria digitata</italic>, and to explore the suitability of applying the optimum UAE conditions for <italic>L. digitata</italic> to other brown macroalgae (<italic>L. hyperborea</italic> and <italic>Ascophyllum nodosum</italic>). The RSM with three-factor, four-level Box-Behnken Design (BBD) was used to study and optimize the extraction variables. A second order polynomial model fitted well to the experimental data with R2 values of 0.79, 0.66, 0.64, 0.73 for fucose, total glucans, FRAP and DPPH, respectively. The UAE parameters studied had a significant influence on the levels of fucose, FRAP and DPPH. The optimised UAE conditions (temperature = 76 °C, time = 10 min and amplitude = 100%) achieved yields of fucose (1060.7 ± 70.6 mg/100 g dried seaweed (ds)), total glucans (968.6 ± 13.3 mg/100 g ds), FRAP (8.7 ± 0.5 µM trolox/mg freeze-dried extract (fde)) and DPPH (11.0 ± 0.2%) in <italic>L. digitata</italic>. Polysaccharide rich extracts were also attained from <italic>L. hyperborea</italic> and <italic>A. nodosum</italic> with variable results when utilizing the optimum UAE conditions for <italic>L. digitata</italic>.
- Subjects
LAMINARIA digitata; EXTRACTION (Chemistry); FUCOSE; GLUCANS; ANTIOXIDANTS; RESPONSE surfaces (Statistics)
- Publication
Marine Drugs, 2018, Vol 16, Issue 8, p257
- ISSN
1660-3397
- Publication type
Article
- DOI
10.3390/md16080257