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- Title
Effect of capilliposide for induction apoptosis in human nasopharyngeal cancer CNE-2 cells through up-regulating PUMA expression.
- Authors
Hua Yonghong; Hu Qiaoying; Piao Yongfeng; Tang Qiu; Feng Jiangguo; Hua, Yonghong; Hu, Qiaoying; Piao, Yongfeng; Tang, Qiu; Feng, Jiangguo
- Abstract
<bold>Objective: </bold>To observe the apoptosis of capilliposide against human nasopharyngeal cancer CNE-2 cells and to study its primary mechanisms.<bold>Materials and Methods: </bold>Vectors pSilencer-PUMA-small interfering RNA (siRNA) were constructed to transcribe functional siRNA specially targeting PUMA. The interfering plasmids were used to transfect CNE-2 cells with lipofectamine 2000 transfection reagent. PUMA messenger RNA (mRNA) expression levels were analyzed by polymerase chain reaction. The proliferation of CNE-2 cells was detected using MTT colorimetry. Annexin V/propidium iodide double staining was applied to detect the apoptosis rate of CNE-2 cells. The protein levels of p53, PUMA, and Bax were detected using Western blot analysis.<bold>Results: </bold>Recombinant siRNA expression vector targeting PUMA was constructed. MTT assays showed capilliposide inhibited the proliferation of CNE-2 cells in a concentration-dependent manner. The inhibition was strengthened along with increased concentrations. Apoptosis detected by flow cytometry in control group, drug group, siRNA group, and drug combined siRNA group was 9.3 ± 2.3%, 31.4 ± 5.6%, 12.3 ± 4.1%, and 13.2 ± 3.7%, respectively. After pretreated by capilliposide, PUMA protein was upregulated, and BAX was distributed to mitochondria in CNE-2 cells using Western blot analysis, but this effect can be interrupted by PUMA-siRNA.<bold>Conclusions: </bold>Capilliposide could induce the apoptosis of CNE-2 cells, which might be related with the increasing in PUMA-Bax pathway.
- Subjects
NASOPHARYNX cancer; CHINESE medicine; APOPTOSIS; LYSIMACHIA; SMALL interfering RNA; P53 antioncogene; CANCER treatment; PROTEIN metabolism; ANTINEOPLASTIC agents; BIOCHEMISTRY; CELL lines; CELL physiology; CLINICAL drug trials; GENE expression; GLYCOSIDES; HYDROCARBONS; PHENOMENOLOGY; NASOPHARYNX tumors; PROTEINS; PHARMACODYNAMICS
- Publication
Journal of Cancer Research & Therapeutics, 2015, Vol 11, pC239
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.170529