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- Title
Identification of Human Cytomegalovirus in placenta, brain, and liver of stillbirth cases.
- Authors
Herrera-Salazar, Alma; Valdespino-Vázquez, María Yolotzin; González-Gallardo, Susana Adelaida; Velasco-Meléndez, Josué Omar; Fonseca-Coronado, Salvador; Moreno-Verduzco, Elsa Romelia
- Abstract
One stillbirth occurs every 16 seconds worldwide. Despite medical advances, infection contributes to 10–25 % of stillbirths in high-income countries. In Mexico, during 2008–2019, national databases recorded 198 076 stillbirths and nineteen cases were attributable to viruses, being human cytomegalovirus (HCMV) identified in nine cases. Previously, our team found an HCMV prevalence of 60.65 % in placenta samples of pregnancy women; so, we decided to identify the presence of HCMV in stillbirth. A retrospective, cross sectional, descriptive study was performed. Twenty-four cases of stillbirth of the National Institute of Perinatology were selected. DNA was extracted from placenta, brain and liver using a commercial kit. To detect the DNA HCMV, a nested PCR was performed to amplify 219 base pairs of the UL123 gene. GAPDH gene was used as integrity control of DNA. DNA HCMV was detected in 14/23 (61 %) of the cases studied. The necropsy files of positive cases reported clinico-pathological findings of congenital HCMV infections as corioamnionitis, villitis, hydrops fetalis not due to immune disease. This study highlights the importance of implement detection of viral infection as causative of stillbirth. We recommend studying a higher number of cases and identify the HCMV genotypes to analyze the correlation between the genotypes and clinico-pathological findings.
- Publication
Veterinaria México OA, 2024, Vol 11, p75
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305