We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Validation of the Chemical and Biological Steps Required Implementing an Advanced Multi-Omics Approach for Assessing the Fate and Impact of Contaminants in Lagoon Sediments.
- Authors
Mejait, Anouar; Fildier, Aurélie; Giroud, Barbara; Daniele, Gaëlle; Wiest, Laure; Raviglione, Delphine; Kotarba, Jules; Toulza, Eve; Ramirez, Triana; Lanseman, Alexia; Clerissi, Camille; Vulliet, Emmanuelle; Calvayrac, Christophe; Salvia, Marie-Virginie
- Abstract
The increasing use of chemicals requires a better understanding of their presence and dynamics in the environment, as well as their impact on ecosystems. The aim of this study was to validate the first steps of an innovative multi-omics approach based on metabolomics and 16S metabarcoding data for analyses of the fate and impact of contaminants in Mediterranean lagoons. Semi-targeted analytical procedures for water and sediment matrices were implemented to assess chemical contamination of the lagoon: forty-six compounds were detected, 28 of which could be quantified in water (between 0.09 and 47.4 ng/L) and sediment (between 0.008 and 26.3 ng/g) samples using the UHPLC-MS/MS instrument. In addition, a non-targeted approach (UHPLC-HRMS) using four different sample preparation protocols based on solid/liquid extractions or an automated pressurized fluid extraction system (EDGE®) was carried out to determine the protocol with the best metabolome coverage, efficiency and reproducibility. Solid/liquid extraction using the solvent mixture acetonitrile/methanol (50/50) was evaluated as the best protocol. Microbial diversity in lagoon sediment was also measured after DNA extraction using five commercial extraction kits. Our study showed that the DNeasy PowerSoil Pro Qiagen kit (Promega, USA) was the most suitable for assessing microbial diversity in fresh sediment.
- Subjects
BACTERIAL diversity; MICROBIAL diversity; MULTIOMICS; LAGOONS; ACETONITRILE
- Publication
Metabolites (2218-1989), 2024, Vol 14, Issue 8, p454
- ISSN
2218-1989
- Publication type
Article
- DOI
10.3390/metabo14080454