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- Title
Redox regulation of ascorbate and glutathione by a chloroplastic dehydroascorbate reductase is required for high-light stress tolerance in Arabidopsis.
- Authors
Noshi, Masahiro; Hatanaka, Risa; Tanabe, Noriaki; Terai, Yusuke; Maruta, Takanori; Shigeoka, Shigeru
- Abstract
Chloroplasts are a significant site for reactive oxygen species production under illumination and, thus, possess a well-organized antioxidant system involving ascorbate. Ascorbate recycling occurs in different manners in this system, including a dehydroascorbate reductase (DHAR) reaction. We herein investigated the physiological significance of DHAR3 in photo-oxidative stress tolerance inArabidopsis. GFP-fused DHAR3 protein was targeted to chloroplasts inArabidopsisleaves. A DHAR3 knockout mutant exhibited sensitivity to high light (HL). Under HL, the ascorbate redox states were similar in mutant and wild-type plants, while total ascorbate content was significantly lower in the mutant, suggesting that DHAR3 contributes, at least to some extent, to ascorbate recycling. Activation of monodehydroascorbate reductase occurred indhar3mutant, which might compensate for the lack of DHAR3. Interestingly, glutathione oxidation was consistently inhibited indhar3mutant. These findings indicate that DHAR3 regulates both ascorbate and glutathione redox states to acclimate to HL. The redox regulation of ascorbate and glutathione by a chloroplastic dehydroascorbate reductase (DHAR3) is essential for photo-oxidative stress tolerance inArabidopsis.
- Subjects
ASCORBATE oxidase; ARABIDOPSIS thaliana; PHOTOOXIDATIVE stress; PHYSIOLOGY
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 5, p870
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2015.1135042