We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cytoprotective Encapsulation of Individual Jurkat T Cells within Durable TiO<sub>2</sub> Shells for T-Cell Therapy.
- Authors
Youn, Wongu; Ko, Eun Hyea; Kim, Mi ‐ Hee; Park, Matthew; Hong, Daewha; Seisenbaeva, Gulaim A.; Kessler, Vadim G.; Choi, Insung S.
- Abstract
Lymphocytes, such as T cells and natural killer (NK) cells, have therapeutic promise in adoptive cell transfer (ACT) therapy, where the cells are activated and expanded in vitro and then infused into a patient. However, the in vitro preservation of labile lymphocytes during transfer, manipulation, and storage has been one of the bottlenecks in the development and commercialization of therapeutic lymphocytes. Herein, we suggest a cell-in-shell (or artificial spore) strategy to enhance the cell viability in the practical settings, while maintaining biological activities for therapeutic efficacy. A durable titanium oxide (TiO2) shell is formed on individual Jurkat T cells, and the CD3 and other antigens on cell surfaces remain accessible to the antibodies. Interleukin-2 (IL-2) secretion is also not hampered by the shell formation. This work suggests a chemical toolbox for effectively preserving lymphocytes in vitro and developing the lymphocyte-based cancer immunotherapy.
- Subjects
TITANIUM oxides; CYTOPROTECTION; CELLULAR therapy; LYMPHOCYTES; CELL survival; CANCER immunotherapy
- Publication
Angewandte Chemie, 2017, Vol 129, Issue 36, p10842
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201703886