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- Title
Development and identification of a wheat- Leymus mollis multiple alien substitution line.
- Authors
Zhao, Ji-Xin; Du, Wan-Li; Wu, Jun; Cheng, Xue-Ni; Gao, Yang; Pang, Yu-Hui; Chen, Xin-Hong; Liu, Shu-Hui; Yang, Qun-Hui; Fu, Jie
- Abstract
Leymus mollis (Trin.) Pilger is resistant to drought, cold, and many fungal and bacterial diseases, and it grows well on barren and salty soils. It has short, strong stems and large spikes with multiple spikelets. It provides an excellent material for wheat ( Triticum aestivum L.) improvement. An octoploid Tritileymus line M842-12 was crossed with T. durum L. cv. Trs-372 and among F5 progenies screened a multiple alien substitution line (05DM6) was identified. This line has 2 n = 42 chromosomes and forms 21 bivalents in the metaphase I of meiosis. Genomic in situ hybridization analysis of the root tips and pollen mother cells indicated that 05DM6 carried 36 chromosomes from wheat and six chromosomes from L. mollis, and these six, presumed to be from the Ns genome, formed three bivalents. DNA marker analysis showed that relative to wheat, 05DM6 lost chromosomes 1, 5, and 6 from the D-genome but retained chromosomes 2, 3, 4, and 7, implying that the retained L. mollis chromosomes belong to homoeologous groups 1, 5 and 6. Electrophoretic analysis of the storage proteins confirmed that a group 1 homoeologue from L. mollis was present in 05DM6. The 05DM6 substitution line has reduced height relative to its parents and is remarkably resistant to stripe rust. This multiple alien substitution line can be employed as a bridge parent in wheat breeding and chromosome engineering.
- Subjects
WHEAT genetics; PLANT chromosomes; GENETIC markers; PLANT genomes; PLANT hybridization; STRIPE rust; DISEASE resistance of plants
- Publication
Euphytica, 2013, Vol 190, Issue 1, p45
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-012-0772-3