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- Title
Two microsatellite markers flanking a dominant gene for resistance to soybean cust nematode race 3.
- Authors
Cervigni, Gerardo Domingo Lucio; Schuster, Ivan; de Barros, Everaldo Gonçalves; Moreira, Maurílio Alves
- Abstract
The purpose of this work was to identify microsatellite markers linked to a gene for resistance to Heterodera glycines Ichinohe (Soybean Cyst Nematode – SCN) in soybean cultivar Hartwig. A BC1F2 mapping population derived from a cross between Hartwig (resistant) and the Brazilian soybean line Y23 (susceptible) was used. About 200 microsatellite or simple sequence repeat (SSR) primer pairs were tested in a bulked segregant analysis (BSA). Those that showed clear polymorphisms were amplified in the BC1F2 population, which had been previously inoculated and evaluated for resistance/susceptibility to SCN Race 3. Three SSR markers linked to SCN resistance were detected in the population. Two of them, Satt 038 and Satt 163, flanking a dominant resistant gene (d/a = –0.90), explained 37% of the phenotypic variance. This gene was mapped at the edge of molecular linkage group G. Broad and narrow sense heritabilities were estimated to be 50.54% and 37.73%, respectively. A selection efficiency of 91.18% was obtained with the simultaneous use of the two markers. The identified SSR markers will be useful tools for assisting the selection of homozygous genotypes and for expediting the introgression of the SCN resistance locus from cv. Hartwig to soybean elite cultivars.
- Subjects
SOYBEAN cyst nematode; MICROSATELLITE repeats; PLANT genetics; SOYBEAN; CULTIVARS; PLANT population genetics
- Publication
Euphytica, 2004, Vol 135, Issue 1, p99
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1023/B:EUPH.0000009545.87679.a1