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- Title
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with high molar fractions of 3-hydroxyvalerate by a threonine-overproducing mutant of Alcaligenes sp. SH-69.
- Authors
Gang Guk Choi; Moo Woong Kim; Jeong-Yoon Kim; Young Ha Rhee
- Abstract
A threonine overproducing mutant of Alcaligenes sp. SH-69 was isolated and its ability to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3HB-co-3HV), was investigated. The 3HV fraction in poly(3HB-co-3HV) produced from glucose as the sole carbon source exceeded 22 mol%, which is approximately six times higher than that achieved by the wild type under the same culture conditions. Furthermore, the addition of a relatively low concentration (10 mM) of propionic acid, valeric acid or levulinic acid to the glucose medium greatly increased the molar fraction of 3HV in the copolyester, to 38–77 mol%. The results suggest that metabolic engineering of the biosynthetic pathways supplying polyhydroxyalkanoate monomers, such as the threonine biosynthetic pathway, can lead to new poly(3HB-co-3HV)-producing strains.
- Subjects
POLY-beta-hydroxybutyrate; GABA derivatives; GRAM-negative bacteria; SUCROSE; FATTY acids; PROPIONIC acid
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 9, p665
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1023437013044