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- Title
Diazepam metabolism and albumin secretion of porcine hepatocytes in collagen-sandwich after cryopreservation.
- Authors
Karim, Najibulla; Allmeling, Christina; Hengstler, Jan-Georg; Haverich, Axel; Bader, Augustinus
- Abstract
Cryopreserved porcine hepatocytes in collagen cultures secreted albumin (up to 98 ± 3 μg ml-1; non-cryopreserved controls: 100 ± 9 μg ml-1) and metabolised diazepam (67.5% ± 7.5% of initially applied diazepam are metabolised; 77.5% ± 10% in non-cryopreserved controls) for up to 14 days after thawing. The cultures resembled the recently developed flat membrane bioreactors. Addition of 5% (v/v) serum did not effect diazepam metabolism. Hepatocytes in collagen-sandwich cultures offer a storable liver support system for potential clinical use.
- Subjects
CRYOPRESERVATION of organs, tissues, etc.; LIVER cells; COLLAGEN; ALBUMINS; DIAZEPAM; BIOREACTORS
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 20, p1647
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005685912195