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- Title
Improve the viability and extracellular polymeric substances bioactivity of Lactiplantibacillus plantarum VAL6 using the environmental adaptation.
- Authors
Phu-Tho Nguyen; Thi-Tho Nguyen; Thi-To-Uyen Nguyen; Quoc-Khanh Hoangf; Huu-Thanh Nguyen
- Abstract
Environmental challenges including high temperature, acidic or alkaline pH, sodium chloride, and increased carbon dioxide concentration, had different impacts on the production, the properties of extracellular polymeric substances (EPS), and the viability after freeze-drying of Lactiplantibacillus plantarum VAL6. Those influences were observed and thoroughly analyzed in this study for the purpose of demonstrating that the bioactivity and amount of EPS related directly to the improvement in survival of lactic acid bacteria (LAB) during freeze-drying. The results showed that these treatments could stimulate L. plantarum VAL6 to produce EPS at different levels resulting in improved viability of the strain. In particular, stress at pH 3.5 caused the highest increase in EPS synthesis (2.3 fold higher than the control), corresponding to the highest survival factor (1.73 fold higher than the control). A model for the experimental relationship between the levels of synthesized EPS and the survival factor of the strain was represented by the equation: y = −0.0463x² + 0.5114x − 0.4565 with R² = 0.9635. According to an analysis of EPS properties, it was found that the tested environmental challenges strongly increased the 1,1-dihpenyl-2-picrylhydrazyl radical scavenging activity and total antioxidant capacity of synthesized EPS. These findings indicated that the antioxidant properties and content of EPS play a key role in improving the freeze-dried viability of LAB.
- Subjects
OXIDANT status; MICROBIAL exopolysaccharides; LACTIC acid bacteria; FREEZE-drying; CARBON dioxide; SALT
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2022, Vol 131, p149
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2021.11.006