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- Title
Nanodiamonds as an effective and novel matrix for immobilizing β galactosidase.
- Authors
Ansari, Shakeel Ahmed; Satar, Rukhsana; Zaidi, Syed Kashif; Naseer, Muhammad Imran; Karim, Sajjad; Alqahtani, Mohammad Husein; Rasool, Mahmood
- Abstract
The present study demonstrates the biospecific adsorption of Aspergillus oryzae β galactosidase on glutaraldehyde functionalized nanodiamonds (NDs). Transmission electron microscopy showed that the synthesized NDs were of 20 nm size. Enzyme activity retained as a result of immobilization was 7420 U/gm of modified NDs. The optimal pH and temperature for soluble and immobilized β galactosidase was observed at pH 4.5 and at 50 °C, respectively. However, significant stability was observed at both higher and lower limits of pH and temperature for the enzyme immobilized on glutaraldehyde modified NDs. Moreover, our findings demonstrated that β galactosidase immobilized on surface functionalized NDs retained greater biocatalytic activity even after 2 months of storage and at higher galactose concentration, and upon repeated uses as compared to enzyme in solution. Modified NDs bound β galactosidase showed improved hydrolysis of lactose from solution in batch processes at various temperatures even after 10 h, thereby suggesting its use for hydrolyzing lactose in dairy products.
- Subjects
NANODIAMONDS; KOJI; BIOPROSTHESIS; LACTOSE products; DAIRY products
- Publication
Food & Bioproducts Processing: Transactions of the Institution of Chemical Engineers Part C, 2015, Vol 95, p298
- ISSN
0960-3085
- Publication type
Article
- DOI
10.1016/j.fbp.2014.10.014