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- Title
Comparison of susceptibility to SIVmac239 infection between CD4<sup>+</sup> and CD4<sup>+</sup>8<sup>+</sup> T cells.
- Authors
Takahashi, M.; Ido, E.; Uesaka, H.; Fukushima, T.; Ibuki, K.; Miura, T.; Hayami, M.; Takahashi, H.
- Abstract
CD4-bearing T cells are the primary targets for human immunodeficiency virus type 1(HIV-1)/simian immunodeficiency virus (SIV) infection. However, it is unclear whether the susceptibility of CD4-bearing T cells including CD4 single positive and CD4/8 double positive T cells to HIV/SIV infection is the same or not. In this study, we compared the susceptibility to SIV infection between CD4+ and CD4+8+ T cells, using Herpesvirus saimiri (HVS)-transformed CD4+ and CD4+8+ T cells established from peripheral blood mononuclear cells (PBMC) of rhesus macaques. Although there was little difference between the two CD4-bearing T cell population in the expression level of CD4 molecules and chemokine receptors such as CXCR4 and CCR5, SIV replicated more efficiently in CD4+8+ T cells than in CD4+ T cells. Moreover, we found that reverse transcription initiated more efficiently in CD4+8+ T cells than in CD4+ T cells and that the cell lysates from CD4+ T cells impaired the RT activity more strongly than that from CD4+8+ T cells. These findings suggest that intracellular environment in CD4+ 8+ T cells is better for reverse transcription and that the infection of those CD4+8+ T cells might play critical and different roles in HIV-1/SIV infection and dissemination.
- Subjects
T cells; CD4 antigen; CD antigens; GLYCOPROTEINS; IMMUNODEFICIENCY; VIRUSES; HIV infections
- Publication
Archives of Virology, 2005, Vol 150, Issue 8, p1517
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-005-0536-7