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- Title
Modulation of Caenorhabditis elegans immune response and modification of Shigella endotoxin upon interaction.
- Authors
Kesika, Periyanaina; Prasanth, Mani Iyer; Balamurugan, Krishnaswamy
- Abstract
To analyze the pathogenesis at both physiological and molecular level using the model organism, Caenorhabditis elegans at different developmental stages in response to Shigella spp. and its pathogen associated molecular patterns such as lipopolysaccharide. The solid plate and liquid culture-based infection assays revealed that Shigella spp. infects C. elegans and had an impact on the brood size and pharyngeal pumping rate. LPS of Shigella spp. was toxic to C. elegans. qPCR analysis revealed that host innate immune genes have been modulated upon Shigella spp. infections and its LPS challenges. Non-destructive analysis was performed to kinetically assess the alterations in LPS during interaction of Shigella spp. with C. elegans. The modulation of innate immune genes attributed the surrendering of host immune system to Shigella spp. by favoring the infection. LPS appeared to have a major role in Shigella-mediated pathogenesis and Shigella employs a tactic behavior of modifying its LPS content to escape from the recognition of host immune system.
- Subjects
CAENORHABDITIS elegans genetics; IMMUNE response; SHIGELLA; ENDOTOXIN analysis; IMMUNE system; GENETICS
- Publication
Journal of Basic Microbiology, 2015, Vol 55, Issue 4, p432
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.201400511