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- Title
Factors affecting plate assay of gentamicin I Diluents.
- Authors
Shanson, D. C.; Daniels, J. V.
- Abstract
The diluent for making up standards for assay of serum or plasma gentamicin was found to be of major importance. When phosphate buffers were used errors of from +33 to 100% occurred depending on the pH and molarity of the buffer and the concentration of gentamicin in the standard. Sodium phosphate antagonized gentamicin, markedly increasing the MIC to gentamicin of the assay strain NCTC 10896. The use of Barbitone buffer as a diluent also produce significant errors, reducing values of gentamicin in test sera in contrast to the raised values obtained with phosphate buffers. No significant differences were found when horse serum, human serum or human plasma was used as a diluent for standards of 1 £g/ml or above. Standards made in acid serum also produced errors, more marked in Difco 11 medium than in Sensitest agar. Changes of pH from 7.5 to 8.3 had little effect on serum standards and all samples of stored horse or human sara tested were found to be within this pH range.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1975, Vol 1, Issue 2, p219
- ISSN
0305-7453
- Publication type
Article